Nepal: The first
phase of research started by Nepali researchers on the effectiveness of herbs
in the treatment of corona infections is nearing completion. The work of
computational chemistry (molecular docking and simulation) of the research led
by Ram Chandra Basnyat has reached its final stage.
Researchers
raised the number of compounds to 500 after only 136 of the compounds (molecules)
found in herbs used by Corona infected were to be studied on the basis of
computer chemistry.
The
professor and researcher of the department, Dr. Niranjan Parajuli said, "We
were told to test only 136 molecules. But as the investigation progressed, the
number reached 500. '
Although 136
experiments identified plants, other compounds were selected on the basis of
reports. "We estimated that these 500 compounds could be potential drugs,"
said Prof. Dr. Parajuli said, "26 molecules have been identified from
there."
Some of
these identified compounds are available in the market. Antibodies are formed
from these molecules. Basnyat said. With the help of technology, there will
also be a test on whether the spike protein of the virus binds or not. "We
have bought spike proteins because of the mutations in spike proteins that have
caused many different variants," he said. "That's why we bought Spike
Protein," said Parajuli. Weight labs test whether the spike binds to the
molecules in the protein. '
The 26 identified
chemicals will be 'treated'. Weight labs will then test whether the corona
spikes go into the protein and bind. The rats are then tested to make
antibodies from those confirmed.
The mice
being tested are of the Balbabi / C type. In the Swiss albino found in Nepal, it is
not possible to make endibody. Parajuli said. He said that Balbabi / C mice
should be brought from Singapore
and India.
He said that the delay was due to the provision that these mice should be kept
in quarantine. Parajuli informed that they have already informed the concerned
bodies and suppliers about this.
First we
make antibodies from mice that are not covid. The antibody will be tested to
see if it works in covid mice. He said 60 to 100 rats would be used during the
research.
The
research was conducted by the Department of Chemistry in collaboration with the
University Grants Commission for covid test. To complete this research, 32 core
computers and software have been procured.
A bio-safety cabinet has been constructed in
the lab. A freezer of minus 86 degrees is also kept. This double-door lab
includes other research instruments, including the invert microscope. Bio-safety
cabinet has been made in this lab of BSL-2 level. The CO2 incubator required
for cell culture is also connected. One lab has all the equipment needed to
make antibodies, he said. The Department of Chemistry has stated that Rs 5
million has been spent to build the lab alone.
A weight
lab is a specialized laboratory where important types of research work are
performed. This lab is rarely used in other departments of Tribhuvan University.
Parajuli has a claim.
0 Comments